Marble Burying Test Autism

In the current study significant differences in marble burying behavior were observed between pm 2 5 exposed and control rats figure 1b and supplementary figure 3 f 3 35 37 56 p 001.

Marble burying test autism.

The marble burying test is a useful model of both obsessive compulsive like ocd behavior and or anxiety like behavior. The marble burying test is commonly used to detect repetitive digging behavior servadio et al 2015. The marble burying test occurred 8 days following the grooming test to ensure there were no potential residual effects from the initial drug treatment birdsall et al 1978. For the communication domain neonatal ultrasonic vocalization and olfactory habituation test toward social and non social odor are described.

Choi et al 2016. Marble burying is an animal model used in scientific research to depict anxiety or obsessive compulsive disorder ocd behavior. Subjects received a different treatment before marble burying from that administered during the spontaneous self grooming experiment with the exception of three b6 mice. Santini et al 2013.

The marble burying test protocol described below is modified from deacon 2006 and angoa perez kane. In this unit behavioral tests are described that are relevant to the domains affected in asd. It is based on the observation that rats and mice will bury either harmful or harmless objects in their bedding. Each mouse was placed for 45 min into a clean rat cage with 4 cm deep bedding and 12 glass marbles placed on the surface.

The marble burying test takes advantage of the proclivity of mice to dig in natural settings e g. While widely used there is significant controversy over the interpretation of its results. The number of marbles that were buried by the mouse was measured. The setup for the marble burying test consists of a standard mouse housing cage filled with a thick layer 5 cm of shaved aspen bedding.

When put in a cage with marbles mice with ocd like symptoms tend to engage in a high degree of repetitive behaviors including digging while mice with a high degree of anxiety tend to engage in a high degree of digging in novel contexts potentially as a means to attempt. For the repetitive domain the t maze spontaneous alternation test and marble burying test are described. If a transparent ceiling with breathing holes is placed on top of testing cage this will prevent the test subject from leaving the cage while allowing unrestricted video recording.